Anti-phospho-DBC1 (Thr454)

Analysis Note

ControlUntreated and etoposide treated Jurkat cell lysates

Application

Peptide Inhibition Assay: A 1:500 dilution from a representative lot blocked DBC-1 phosphorylated at Thr454 in untreated and etoposide treated Jurkat cell lysates.

Research CategorySignaling

Anti-phospho-DBC1 (Thr454) Antibody is an antibody against phospho-DBC1 (Thr454) for use in Western Blotting, Peptide Inhibition Assay.

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Deleted in breast cancer gene 1 protein (DBC1) is a large, nuclear protein with multiple functions in cell survival that binds directly to the estrogen receptor α (ERα) hormone-binding domain in a strict ligand-independent manner. DBC1 promotes p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity. It is ubiquitously expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.

Immunogen

KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454.

Epitope: Phosphorylated Thr454

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in untreated and etoposide treated Jurkat cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DBC-1 phosphorylated at Thr454 in 10 µg of etoposide treated Jurkat cell lysates.

Specificity

This antibody recognizes DBC1 phosphorylated at Thr454.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed. Uniprot states the calculated molecular weight is 103 kDa but has been shown at ~130kDa (Satoh, M., et al. (1995). Brain Res Dev Brain Res. 87(2):111-119).